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expression vector used was pet-30a(+)  (Millipore)


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    Structured Review

    Millipore expression vector used was pet-30a(+)
    Expression Vector Used Was Pet 30a(+), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/expression+vector+used+was+pet-30a(+)/pet28a/us11667896-231-69-75
    Average 90 stars, based on 1 article reviews
    expression vector used was pet-30a(+) - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Modified DAAO enzyme and application thereof
    Article Snippet: The particular gene cloning operation can be seen in the aforementioned Sambrook et al., 1989. i) Reagents: DNA polymerase (Prime STAR Max DNA Polymerase) and DpnI endonuclease were purchased from TaKaRa; plasmid isolation kit was purchased from Axygen; catalase was purchased from Zaozhuang Quanding Biological Technology Co., Ltd., item number QD-001; and D,L-Glufosinate was purchased from Lier Chemical Co., Ltd. ii) Vectors and Strains: The expression vector used was pET-30a(+), the plasmid was purchased from Novagen; and the host cell used was E. coli BL21(DE3), purchased from Tiangen BioTech (Beijing) Co., Ltd. iii) Sequencing and Primer Synthesis were Accomplished by Synbio Technologies Co., Ltd. iv) Site-Directed Mutation: Specific primer pairs were designed to introduce the desired substitutions at the bases corresponding to the amino acid positions that are needed to be mutated.

    Plasmid Preparation:

    Article Title: Modified DAAO enzyme and application thereof
    Article Snippet: The particular gene cloning operation can be seen in the aforementioned Sambrook et al., 1989. i) Reagents: DNA polymerase (Prime STAR Max DNA Polymerase) and DpnI endonuclease were purchased from TaKaRa; plasmid isolation kit was purchased from Axygen; catalase was purchased from Zaozhuang Quanding Biological Technology Co., Ltd., item number QD-001; and D,L-Glufosinate was purchased from Lier Chemical Co., Ltd. ii) Vectors and Strains: The expression vector used was pET-30a(+), the plasmid was purchased from Novagen; and the host cell used was E. coli BL21(DE3), purchased from Tiangen BioTech (Beijing) Co., Ltd. iii) Sequencing and Primer Synthesis were Accomplished by Synbio Technologies Co., Ltd. iv) Site-Directed Mutation: Specific primer pairs were designed to introduce the desired substitutions at the bases corresponding to the amino acid positions that are needed to be mutated.

    Isolation:

    Article Title: Modified DAAO enzyme and application thereof
    Article Snippet: The particular gene cloning operation can be seen in the aforementioned Sambrook et al., 1989. i) Reagents: DNA polymerase (Prime STAR Max DNA Polymerase) and DpnI endonuclease were purchased from TaKaRa; plasmid isolation kit was purchased from Axygen; catalase was purchased from Zaozhuang Quanding Biological Technology Co., Ltd., item number QD-001; and D,L-Glufosinate was purchased from Lier Chemical Co., Ltd. ii) Vectors and Strains: The expression vector used was pET-30a(+), the plasmid was purchased from Novagen; and the host cell used was E. coli BL21(DE3), purchased from Tiangen BioTech (Beijing) Co., Ltd. iii) Sequencing and Primer Synthesis were Accomplished by Synbio Technologies Co., Ltd. iv) Site-Directed Mutation: Specific primer pairs were designed to introduce the desired substitutions at the bases corresponding to the amino acid positions that are needed to be mutated.

    Expressing:

    Article Title: Modified DAAO enzyme and application thereof
    Article Snippet: The particular gene cloning operation can be seen in the aforementioned Sambrook et al., 1989. i) Reagents: DNA polymerase (Prime STAR Max DNA Polymerase) and DpnI endonuclease were purchased from TaKaRa; plasmid isolation kit was purchased from Axygen; catalase was purchased from Zaozhuang Quanding Biological Technology Co., Ltd., item number QD-001; and D,L-Glufosinate was purchased from Lier Chemical Co., Ltd. ii) Vectors and Strains: The expression vector used was pET-30a(+), the plasmid was purchased from Novagen; and the host cell used was E. coli BL21(DE3), purchased from Tiangen BioTech (Beijing) Co., Ltd. iii) Sequencing and Primer Synthesis were Accomplished by Synbio Technologies Co., Ltd. iv) Site-Directed Mutation: Specific primer pairs were designed to introduce the desired substitutions at the bases corresponding to the amino acid positions that are needed to be mutated.

    Sequencing:

    Article Title: Modified DAAO enzyme and application thereof
    Article Snippet: The particular gene cloning operation can be seen in the aforementioned Sambrook et al., 1989. i) Reagents: DNA polymerase (Prime STAR Max DNA Polymerase) and DpnI endonuclease were purchased from TaKaRa; plasmid isolation kit was purchased from Axygen; catalase was purchased from Zaozhuang Quanding Biological Technology Co., Ltd., item number QD-001; and D,L-Glufosinate was purchased from Lier Chemical Co., Ltd. ii) Vectors and Strains: The expression vector used was pET-30a(+), the plasmid was purchased from Novagen; and the host cell used was E. coli BL21(DE3), purchased from Tiangen BioTech (Beijing) Co., Ltd. iii) Sequencing and Primer Synthesis were Accomplished by Synbio Technologies Co., Ltd. iv) Site-Directed Mutation: Specific primer pairs were designed to introduce the desired substitutions at the bases corresponding to the amino acid positions that are needed to be mutated.

    Mutagenesis:

    Article Title: Modified DAAO enzyme and application thereof
    Article Snippet: The particular gene cloning operation can be seen in the aforementioned Sambrook et al., 1989. i) Reagents: DNA polymerase (Prime STAR Max DNA Polymerase) and DpnI endonuclease were purchased from TaKaRa; plasmid isolation kit was purchased from Axygen; catalase was purchased from Zaozhuang Quanding Biological Technology Co., Ltd., item number QD-001; and D,L-Glufosinate was purchased from Lier Chemical Co., Ltd. ii) Vectors and Strains: The expression vector used was pET-30a(+), the plasmid was purchased from Novagen; and the host cell used was E. coli BL21(DE3), purchased from Tiangen BioTech (Beijing) Co., Ltd. iii) Sequencing and Primer Synthesis were Accomplished by Synbio Technologies Co., Ltd. iv) Site-Directed Mutation: Specific primer pairs were designed to introduce the desired substitutions at the bases corresponding to the amino acid positions that are needed to be mutated.

    Introduce:

    Article Title: Modified DAAO enzyme and application thereof
    Article Snippet: The particular gene cloning operation can be seen in the aforementioned Sambrook et al., 1989. i) Reagents: DNA polymerase (Prime STAR Max DNA Polymerase) and DpnI endonuclease were purchased from TaKaRa; plasmid isolation kit was purchased from Axygen; catalase was purchased from Zaozhuang Quanding Biological Technology Co., Ltd., item number QD-001; and D,L-Glufosinate was purchased from Lier Chemical Co., Ltd. ii) Vectors and Strains: The expression vector used was pET-30a(+), the plasmid was purchased from Novagen; and the host cell used was E. coli BL21(DE3), purchased from Tiangen BioTech (Beijing) Co., Ltd. iii) Sequencing and Primer Synthesis were Accomplished by Synbio Technologies Co., Ltd. iv) Site-Directed Mutation: Specific primer pairs were designed to introduce the desired substitutions at the bases corresponding to the amino acid positions that are needed to be mutated.



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